Abstract and Poster for CaNCURE Day:
Nano-plasmonic exosome (nPLEX) assays for exosome analysis
Craig Pille, Hyungsoon Im, Cesar Castro
Bouvė College of Health Sciences, Northeastern University, and the Center for Systems Biology at Massachusetts General Hospital
Exosomes are phospholipid nanovesicles secreted by mammalian cells that have recently become a topic of interest in cancer research and reports. These miniscule particles are released in large numbers by most types of cancers and often carry molecular information about the parent tumor cell: information that could be useful in diagnostic and tumor response to therapy situations. However, current methods of exosome analysis tend to be difficult to use in clinical settings where exosome concentration is low, or that require high throughput. A group of researchers at the Center for Systems Biology at Massachusetts General Hospital has recently developed a surface plasmon resonance (SPR) assay based on optical transmission through periodic nanoholes called the nano-plasmonic exosome (nPLEX) assay. This generation of the nPLEX sensor was designed for label-free detection of exosomes and validated across different ovarian cancer cell lines and ascites samples from ovarian cancer patients. Using a microarray spotting technology, a next generation nPLEX sensor is being created with the goal of establishing a higher throughput method for exosome detection. The clinical utility of this sensor is being validated in an ongoing proof of concept study utilizing pancreatic cancer clinical samples. During this project, I designed the microarray spotting program for higher nPLEX throughput and ran all clinical samples processed to date in the proof of concept study.