Memorandum
Date: June 11, 2012
To: Future ChemDiscovery Co-op Student
From: Faraz Arastu
Subject: Glen-Pak DNA Purification Protocol
The modified Glen-Pak DNA Purification procedure was approved by the discovery team to replace the previous method, which was time-consuming, costly, and inefficient. This procedure is based on the original protocol published by Glen Research for their Glen-Pak 3g cartridge. The published protocol is not validated for a 5umol scale of DNA synthesized in reverse. However, the modified protocol is designed to cost-effectively purify a 5umol scale synthesis of Reverse-DNA in high yield.
ChemDiscovery uses a patented DNA-labeling method to identify the compounds they synthesize. The higher the yield of pure DNA for labeling, the more compounds the chemistry team can synthesize and test to create potential drug candidates. This procedure is meant to be used cyclically as batches of DNA are made by the DNA synthesis team. It is expected that you will repeat this procedure several times during the first three weeks of your internship, followed by a period of disuse before another three week period of heavy usage.
Throughout the duration of your coop, this protocol will be available to you through the password-protected file server system at the company. You will be able to access the document digitally through the server using Adobe Reader and print copies through the wireless network. However, you will not have administrator access to make changes to the document.
The protocol follows a standard lab procedure format with materials and their corresponding quantities listed first, followed by a sequence of detailed steps for performing the actual purification. The materials section will help you identify and organize all necessary equipment before beginning the procedure. If you are missing any items in the materials section, you should not start the procedure. Please note that, as with all lab practices, proper gloves, lab goggles, and a lab coat must be worn when handling the materials in this protocol. These precautions ensure that no foreign substances contaminate you or the DNA.
Attachment: Glen-Pak Purification of 5umol Scale Reverse-DNA
8 Context Memo [DNA Protocol].pdf